24 March 2016 4D phase-space multiplexing for fluorescent microscopy
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Abstract
Phase-space measurements enable characterization of second-order spatial coherence properties and can be used for digital aberration removal or 3D position reconstruction. Previous methods use a scanning aperture to measure the phase space spectrogram, which is slow and light inefficient, while also attenuating information about higher-order correlations. We demonstrate a significant improvement of speed and light throughput by incorporating multiplexing techniques into our phase-space imaging system. The scheme implements 2D coded aperture patterning in the Fourier (pupil) plane of a microscope using a Spatial Light Modulator (SLM), while capturing multiple intensity images in real space. We compare various multiplexing schemes to scanning apertures and show that our phase-space reconstructions are accurate for experimental data with biological samples containing many 3D fluorophores.
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Hsiou-Yuan Liu, Hsiou-Yuan Liu, Jingshan Zhong, Jingshan Zhong, Laura Waller, Laura Waller, "4D phase-space multiplexing for fluorescent microscopy", Proc. SPIE 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management, 97200A (24 March 2016); doi: 10.1117/12.2213610; https://doi.org/10.1117/12.2213610
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