Selective-plane illumination microscopy for high-content volumetric biological imaging

Ryan McGorty; Bo Huang

doi:10.1117/12.2212889

7 March 2016 Selective-plane illumination microscopy for high-content volumetric biological imaging

Ryan McGorty, Bo Huang

Proceedings Volume 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management; 97200P (2016) https://doi.org/10.1117/12.2212889
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Light-sheet microscopy, also named selective-plane illumination microscopy, enables optical sectioning with minimal light delivered to the sample. Therefore, it allows one to gather volumetric datasets of developing embryos and other light-sensitive samples over extended times. We have configured a light-sheet microscope that, unlike most previous designs, can image samples in formats compatible with high-content imaging. Our microscope can be used with multi-well plates or with microfluidic devices. In designing our optical system to accommodate these types of sample holders we encounter large optical aberrations. We counter these aberrations with both static optical components in the imaging path and with adaptive optics. Potential applications of this microscope include studying the development of a large number of embryos in parallel and over long times with subcellular resolution and doing high-throughput screens on organisms or cells where volumetric data is necessary.

Conference Presentation

Citation Download Citation

Ryan McGorty and Bo Huang "Selective-plane illumination microscopy for high-content volumetric biological imaging", Proc. SPIE 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management, 97200P (7 March 2016); https://doi.org/10.1117/12.2212889

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