7 March 2016 A light sheet confocal microscope for image cytometry with a variable linear slit detector
Author Affiliations +
We present a light sheet confocal microscope (LSCM) capable of high-resolution imaging of cell suspensions in a microfluidic environment. In lieu of conventional pressure-driven flow or mechanical translation of the samples, we have employed a novel method of fluid transport, redox-magnetohydrodynamics (redox-MHD). This method achieves fluid motion by inducing a small current into the suspension in the presence of a magnetic field via electrodes patterned onto a silicon chip. This on-chip transportation requires no moving parts, and is coupled to the remainder of the imaging system. The microscopy system comprises a 450 nm diode 20 mW laser coupled to a single mode fiber and a cylindrical lens that converges the light sheet into the back aperture of a 10x, 0.3 NA objective lens in an epi-illumination configuration. The emission pathway contains a 150 mm tube lens that focuses the light onto the linear sensor at the conjugate image plane. The linear sensor (ELiiXA+ 8k/4k) has three lateral binning modes which enables variable detection aperture widths between 5, 10, or 20 μm, which can be used to vary axial resolution. We have demonstrated redox-MHD-enabled light sheet microscopy in suspension of fluorescent polystyrene beads. This approach has potential as a high-throughput image cytometer with myriad cellular diagnostic applications.
Conference Presentation
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Joshua A. Hutcheson, Joshua A. Hutcheson, Foysal Z. Khan, Foysal Z. Khan, Amy J. Powless, Amy J. Powless, Devin Benson, Devin Benson, Courtney Hunter, Courtney Hunter, Ingrid Fritsch, Ingrid Fritsch, Timothy J. Muldoon, Timothy J. Muldoon, "A light sheet confocal microscope for image cytometry with a variable linear slit detector", Proc. SPIE 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management, 97200U (7 March 2016); doi: 10.1117/12.2211164; https://doi.org/10.1117/12.2211164

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