A study on the characteristics of the Analog Mean Delay (AMD) method for high-speed Fluorescence Lifetime Imaging Microscopy (FLIM)

Byungyeon Kim; Byungjun Park; Seungrag Lee; Youngjae Won

doi:10.1117/12.2209468

7 March 2016 A study on the characteristics of the Analog Mean Delay (AMD) method for high-speed Fluorescence Lifetime Imaging Microscopy (FLIM)

Byungyeon Kim, Byungjun Park, Seungrag Lee, Youngjae Won

Author Affiliations +

Proceedings Volume 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management; 972014 (2016) https://doi.org/10.1117/12.2209468
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We present a study on the characteristics of the AMD method. We have demonstrated that the photon economy of the AMD method is not degraded for longer lifetimes even when the applied integration window size is increased. By an extension of MCS, the photon economy with respect to different designs of the Gaussian low-pass filter (GLPF) used in the AMD setup was also studied. When a GLPF with the highest cutoff frequency of 100 MHz is applied, the most effective photon economy performance is achieved for lifetimes of 1, 3.2, 5, and 8 ns.

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Byungyeon Kim, Byungjun Park, Seungrag Lee, and Youngjae Won "A study on the characteristics of the Analog Mean Delay (AMD) method for high-speed Fluorescence Lifetime Imaging Microscopy (FLIM)", Proc. SPIE 9720, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management, 972014 (7 March 2016); https://doi.org/10.1117/12.2209468

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