22 April 2016 Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules
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Abstract
In this presentation, we explore the feasibility of plasmonic nanohole-based sub-diffraction-limited nanoscopy for biomolecular imaging. The technique utilizes near-field distribution localized by surface plasmon localization on metallic nanoholes which is used to sample molecular fluorescence. The optimum geometry of nanohole arrays was determined by numerical analysis. The localization sampling was applied to reconstructing sub-diffraction-limited images of gliding microtubules with a 76 nm effective resolution in the lateral direction. Extraordinary light transmission was also employed to address enhancement of axial resolution using nanohole arrays, based on which extraction of gliding motions of bacteria was demonstrated with an axial resolution down to 50 nm.
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Wonju Lee, Wonju Lee, Youngjin Oh, Youngjin Oh, Kyujung Kim, Kyujung Kim, Yoshiaki Kinosita, Yoshiaki Kinosita, Nagisa Mikami, Nagisa Mikami, Takayuki Nishizaka, Takayuki Nishizaka, Donghyun Kim, Donghyun Kim, "Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules", Proc. SPIE 9721, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, 972107 (22 April 2016); doi: 10.1117/12.2212903; https://doi.org/10.1117/12.2212903
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