In vitro antitumor efficacy of berberine: solid lipid nanoparticles against human HepG2, Huh7 and EC9706 cancer cell lines

Xiang-Ping Meng; Xiao Wang; Huai-ling Wang; Tong-sheng Chen; Yi-fei Wang; Zhi-ping Wang

doi:10.1117/12.2209448

22 April 2016 In vitro antitumor efficacy of berberine: solid lipid nanoparticles against human HepG2, Huh7 and EC9706 cancer cell lines

Xiang-Ping Meng, Xiao Wang, Huai-ling Wang, Tong-sheng Chen, Yi-fei Wang, Zhi-ping Wang

Author Affiliations +

Proceedings Volume 9722, Colloidal Nanoparticles for Biomedical Applications XI; 97221A (2016) https://doi.org/10.1117/12.2209448
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Hepatocarcinoma and esophageal squamous cell carcinomas threaten human life badly. It is a current issue to seek the effective natural remedy from plant to treat cancer due to the resistance of the advanced hepatocarcinoma and esophageal carcinoma to chemotherapy. Berberine (Ber), an isoquinoline derivative alkaloid, has a wide range of pharmacological properties and is considered to have anti-hepatocarcinoma and antiesophageal carcinoma effects. However its low oral bioavailability restricts its wide application. In this report, Ber loaded solid lipid nanoparticles (Ber-SLN) was prepared by hot melting and then high pressure homogenization technique. The in vitro anti-hepatocarcinoma and antiesophageal carcinoma effects of Ber-SLN relative to efficacy of bulk Ber were evaluated. The particle size and zeta potential of Ber-SLN were 154.3 ± 4.1 nm and −11.7 ± 1.8 mV, respectively. MTT assay showed that Ber-SLN effectively inhibited the proliferation of human HepG2 and Huh7 and EC9706 cells, and the corresponding IC₅₀ value was 10.6 μg/ml, 5.1 μg/ml, and 7.3 μg/ml (18.3μg/ml, 6.5μg/ml, and 12.4μg/ml μg/ml of bulk Ber solution), respectively. These results suggest that the delivery of Ber-SLN is a promising approach for treating tumors.

Citation Download Citation

Xiang-Ping Meng, Xiao Wang, Huai-ling Wang, Tong-sheng Chen, Yi-fei Wang, and Zhi-ping Wang "In vitro antitumor efficacy of berberine: solid lipid nanoparticles against human HepG2, Huh7 and EC9706 cancer cell lines", Proc. SPIE 9722, Colloidal Nanoparticles for Biomedical Applications XI, 97221A (22 April 2016); https://doi.org/10.1117/12.2209448

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