Evaluation of free radical scavenging capacity and antioxidative damage effect of resveratrol-nanostructured lipid carriers

Ju Jin; Fan Shi; Qiu-wen Li; Pei-shan Li; Tong-sheng Chen; Yi-fei Wang; Zhi-ping Wang

doi:10.1117/12.2212464

22 April 2016 Evaluation of free radical scavenging capacity and antioxidative damage effect of resveratrol-nanostructured lipid carriers

Ju Jin, Fan Shi, Qiu-wen Li, Pei-shan Li, Tong-sheng Chen, Yi-fei Wang, Zhi-ping Wang

Author Affiliations +

Proceedings Volume 9722, Colloidal Nanoparticles for Biomedical Applications XI; 97221D (2016) https://doi.org/10.1117/12.2212464
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Cellular damage induced by free-radicals like reactive oxygen species has been implicated in several diseases. 2, 2-azobis(2-amidino-propane) dihydrochloride(AAPH) generates two potent ROS capable of inducing lipid peroxidation: alkoxy radical(RO-) and peroxy radical(ROO-). These radicals are similar to those that are physiologically active and thus might initiate a cascade of intracellular toxic events leading to oxidation, lipid peroxidation, DNA damage and subsequent cell death. Hence naturally anti-oxidant play a vital role in combating these conditions. In this study, resveratrol loaded nanostructured lipid carriers (Res-NLC) was prepared by hot melting and then high pressure homogenization technique. The effects of Res-NLC on free radical scavenging capacity and antioxidative damage is investigated. The particle size and zeta potential of Res-NLC were 139.3 ± 1.7 nm and −11.21 ± 0.41 mV, respectively. By free radical scavenging assays, the IC₅₀ value of Res-NLC were 19.25, 5.29 μg/mL with DPPH, ABTS assay respectively, and 0.161 mg ferrous sulfate/1 mg Res-NLC with FRAP assay; and by AAPH-induced oxidative injury cell model assay, Res-NLC showed the strong protective effect against the human liver tumor HepG2 cell oxidative stress damage. These results indicated that the antioxidant properties of Res-NLC hold great potential used as an alternative to more toxic synthetic antioxidants as an additive in food, cosmetic and pharmaceutical preparations for the oxidative diseases treatment.

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Ju Jin, Fan Shi, Qiu-wen Li, Pei-shan Li, Tong-sheng Chen, Yi-fei Wang, and Zhi-ping Wang "Evaluation of free radical scavenging capacity and antioxidative damage effect of resveratrol-nanostructured lipid carriers", Proc. SPIE 9722, Colloidal Nanoparticles for Biomedical Applications XI, 97221D (22 April 2016); https://doi.org/10.1117/12.2212464

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