Two-photon fluorescent sensor for K+ imaging in live cells (Conference Presentation)

Binglin Sui; Xiling Yue; Bosung Kim; Kevin D. Belfield

doi:10.1117/12.2211202

28 April 2016 Two-photon fluorescent sensor for K⁺ imaging in live cells (Conference Presentation)

Binglin Sui, Xiling Yue, Bosung Kim, Kevin D. Belfield

Proceedings Volume 9723, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII; 972308 (2016) https://doi.org/10.1117/12.2211202
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

It is difficult to overstate the physiological importance of potassium for life as its indispensable roles in a variety of biological processes are widely known. As a result, efficient methods for determining physiological levels of potassium are of paramount importance. Despite this, relatively few K+ fluorescence sensors have been reported, with only one being commercially available. A new two-photon excited fluorescent K+ sensor is reported. The sensor is comprised of three moieties, a highly selective K+ chelator as the K+ recognition unit, a boron-dipyrromethene (BODIPY) derivative modified with phenylethynyl groups as the fluorophore, and two polyethylene glycol chains to afford water solubility. The sensor displays very high selectivity (<52-fold) in detecting K+ over other physiological metal cations. Upon binding K+, the sensor switches from non-fluorescent to highly fluorescent, emitting red to near-IR (NIR) fluorescence. The sensor exhibited a good two-photon absorption cross section, 500 GM at 940 nm. Moreover, it is not sensitive to pH in the physiological pH range. Time-dependent cell imaging studies via both one- and two-photon fluorescence microscopy demonstrate that the sensor is suitable for dynamic K+ sensing in living cells.

Conference Presentation

Citation Download Citation

Binglin Sui, Xiling Yue, Bosung Kim, and Kevin D. Belfield "Two-photon fluorescent sensor for K⁺ imaging in live cells (Conference Presentation)", Proc. SPIE 9723, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII, 972308 (28 April 2016); https://doi.org/10.1117/12.2211202

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