SERS detection and targeted ablation of lymphoma cells using functionalized Ag nanoparticles

Qian Yao; Fei Cao; Chao Feng; Yan Zhao; Xiuhong Wang

doi:10.1117/12.2212285

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22 April 2016 SERS detection and targeted ablation of lymphoma cells using functionalized Ag nanoparticles

Qian Yao, Fei Cao, Chao Feng, Yan Zhao, Xiuhong Wang

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Proceedings Volume 9724, Plasmonics in Biology and Medicine XIII; 972407 (2016) https://doi.org/10.1117/12.2212285
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Lymphoma is a heterogeneous group of malignancies of the lymphoid tissue, and is prevalent worldwide affecting both children and adults with a high mortality rate. There is in dire need of accurate and noninvasive approaches for early detection of the disease. Herein, we report a facile way to fabricate silver nanoparticle based nanoprobe by incorporating the corner-stone immunotherapeutic drug Rituxan for simultaneous detection and ablation of lymphoma cells in vitro. The fabricated nanoprobe can detect CD20 positive single lymphoma cell by surface enhanced Raman scattering technique with high specificity. The engineered nanoprobe retains the same antibody property as intact drug via Antibody-Dependent Cell-mediated Cytotoxicity (ADCC) analysis. The nanoprobe efficiently eradicates lymphoma cells in vitro. By integrating the advantages of sensitive SERS detection with targeted ablation capabilities of immunotherapeutic drug through site specificity, this nanoprobe can be applied as outstanding tools in living imaging, cancer diagnosis and treatment.

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Qian Yao, Fei Cao, Chao Feng, Yan Zhao, and Xiuhong Wang "SERS detection and targeted ablation of lymphoma cells using functionalized Ag nanoparticles", Proc. SPIE 9724, Plasmonics in Biology and Medicine XIII, 972407 (22 April 2016); https://doi.org/10.1117/12.2212285

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