Localized biomolecules immobilization in optical microbubble resonators

S. Berneschi; F. Baldini; A. Barucci; A. Cosci; F. Cosi; D. Farnesi; G. Nunzi Conti; G. C. Righini; S. Soria; S. Tombelli; C. Trono; S. Pelli; A. Giannetti

doi:10.1117/12.2213683

22 April 2016 Localized biomolecules immobilization in optical microbubble resonators

S. Berneschi, F. Baldini, A. Barucci, A. Cosci, F. Cosi, D. Farnesi, G. Nunzi Conti, G. C. Righini, S. Soria, S. Tombelli, C. Trono, S. Pelli, A. Giannetti

Author Affiliations +

Proceedings Volume 9727, Laser Resonators, Microresonators, and Beam Control XVIII; 972719 (2016) https://doi.org/10.1117/12.2213683
Event: SPIE LASE, 2016, San Francisco, California, United States

Abstract

In order to optimize the performance of an optical microbubble resonator (OMBR) as biosensor, the chemical functionalization of its inner surface plays a key role. Here we report on a spatially selective photo – chemical procedure able to bind fluorescent biomolecules only in correspondence of the OMBR inner surface. This abruptly reduces the occurrence of an undesired specific biochemical bond event all along the microfluidic section of the device. The evidence of this method, which maintains high Q factor (> 10⁵) for the OMBR in buffer solution, is proved by fluorescence microscopy and real time measurement of the resonance broadening.

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S. Berneschi, S. Berneschi, F. Baldini, F. Baldini, A. Barucci, A. Barucci, A. Cosci, A. Cosci, F. Cosi, F. Cosi, D. Farnesi, D. Farnesi, G. Nunzi Conti, G. Nunzi Conti, G. C. Righini, G. C. Righini, S. Soria, S. Soria, S. Tombelli, S. Tombelli, C. Trono, C. Trono, S. Pelli, S. Pelli, A. Giannetti, A. Giannetti, "Localized biomolecules immobilization in optical microbubble resonators", Proc. SPIE 9727, Laser Resonators, Microresonators, and Beam Control XVIII, 972719 (22 April 2016); doi: 10.1117/12.2213683; https://doi.org/10.1117/12.2213683

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