High-definition Fourier transform infrared spectroscopic imaging of prostate tissue

Tomasz P. Wrobel; Jin Tae Kwak; Andre Kadjacsy-Balla; Rohit Bhargava

doi:10.1117/12.2217341

23 March 2016 High-definition Fourier transform infrared spectroscopic imaging of prostate tissue

Tomasz P. Wrobel, Jin Tae Kwak, Andre Kadjacsy-Balla, Rohit Bhargava

Proceedings Volume 9791, Medical Imaging 2016: Digital Pathology; 97911D (2016) https://doi.org/10.1117/12.2217341
Event: SPIE Medical Imaging, 2016, San Diego, California, United States

Abstract

Histopathology forms the gold standard for cancer diagnosis and therapy, and generally relies on manual examination of microscopic structural morphology within tissue. Fourier-Transform Infrared (FT-IR) imaging is an emerging vibrational spectroscopic imaging technique, especially in a High-Definition (HD) format, that provides the spatial specificity of microscopy at magnifications used in diagnostic surgical pathology. While it has been shown for standard imaging that IR absorption by tissue creates a strong signal where the spectrum at each pixel is a quantitative “fingerprint” of the molecular composition of the sample, here we show that this fingerprint also enables direct digital pathology without the need for stains or dyes for HD imaging. An assessment of the potential of HD imaging to improve diagnostic pathology accuracy is presented.

Citation Download Citation

Tomasz P. Wrobel, Jin Tae Kwak, Andre Kadjacsy-Balla, and Rohit Bhargava "High-definition Fourier transform infrared spectroscopic imaging of prostate tissue", Proc. SPIE 9791, Medical Imaging 2016: Digital Pathology, 97911D (23 March 2016); https://doi.org/10.1117/12.2217341

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available