Enhancement mechanism of fluorescence intensity in presence of plasmonic nanoparticles

Sumana Das; Ramakrishna Vasireddi; Krishna Harika Villa; Brahmanandam Javvaji; Gopalkrishna M. Hegde; D. Roy Mahapatra

doi:10.1117/12.2207565

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9 December 2015 Enhancement mechanism of fluorescence intensity in presence of plasmonic nanoparticles

Sumana Das, Ramakrishna Vasireddi, Krishna Harika Villa, Brahmanandam Javvaji, Gopalkrishna M. Hegde, D. Roy Mahapatra

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Proceedings Volume 9792, Biophotonics Japan 2015; 979208 (2015) https://doi.org/10.1117/12.2207565
Event: SPIE/OSJ Biophotonics Japan, 2015, Tokyo, Japan

Abstract

The emission intensity of fluorophore molecule may change in presence of strong plasmon field induced by nanoparticles. The enhancement intensity is optimized through selective clustering or functionalization of nanoparticles in closed vicinity of fluorophore. Our study is aimed at understanding the enhancement mechanism of fluorescence intensity in presence of gold nanoparticles to utilize it in molecular sensing and in situ imaging in the microfluidic lab-on-chip device. Related phenomena are studied in situ in a microfluidic channel via fluorescence imaging. Detailed analysis is carried out to understand the possible mechanism of enhancement of fluorescence due to nanoparticles. In the present experimental study we show that SYTO9 fluorescence intensity increased in presence of Au nanoparticles of ~20 nm diameter. The fluorescence intensity is 20 time more compared to that in absence of Au nanoparticles. The enhancement of fluorescence intensity is attributed to the plasmonic resonance of Au nanoparticle at around the fluorescence emission wavelength. Underlying fundamental mechanism via dipole interaction model is explored for quantitative correlation of plasmonic enhancement properties.

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Sumana Das, Ramakrishna Vasireddi, Krishna Harika Villa, Brahmanandam Javvaji, Gopalkrishna M. Hegde, and D. Roy Mahapatra "Enhancement mechanism of fluorescence intensity in presence of plasmonic nanoparticles", Proc. SPIE 9792, Biophotonics Japan 2015, 979208 (9 December 2015); https://doi.org/10.1117/12.2207565

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