A novel microfluidic model to mimic the turbid nature and microvasculature of cutaneous tissue for optical imaging experiments

Chen Chen; Midhat Ahmed; Florian Klämpfl; Florian Stelzle; Michael Schmidt

doi:10.1117/12.2203446

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9 December 2015 A novel microfluidic model to mimic the turbid nature and microvasculature of cutaneous tissue for optical imaging experiments

Chen Chen, Midhat Ahmed, Florian Klämpfl, Florian Stelzle, Michael Schmidt

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Proceedings Volume 9792, Biophotonics Japan 2015; 97920J (2015) https://doi.org/10.1117/12.2203446
Event: SPIE/OSJ Biophotonics Japan, 2015, Tokyo, Japan

Abstract

To provide clinically relevant insights into the device performance of an optical imaging approach to reconstruct the superficial cutaneous micro-circulation (skin angiography), a phantom device with turbid matrix and perfusable micro-vessels is essential. In this work, we describe a novel microfluidic-based device to mimic the micro-vessels and the turbid nature of the epidermis and dermis. This phantom device contains a hollow assay with a diameter of the channels of 50 μm. The hollow assay includes the geometry of the inlet, the river-like assay, and the outlet, which can be perfused by e.g. meta-hemoglobin solution. This imitates the superficial micro-circulation in the skin. The absorption coefficient μ_a and the reduced scattering coefficient μ_s' are adjusted to match those of skin. As an application case, we attempt to reconstruct a 2-D velocity field of the hemoglobin flow in the scattering microfluidic device via the Doppler-mode of an OCT.

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Chen Chen, Midhat Ahmed, Florian Klämpfl, Florian Stelzle, and Michael Schmidt "A novel microfluidic model to mimic the turbid nature and microvasculature of cutaneous tissue for optical imaging experiments", Proc. SPIE 9792, Biophotonics Japan 2015, 97920J (9 December 2015); https://doi.org/10.1117/12.2203446

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