Determination of scattering coefficient considering wavelength and absorption dependence of anisotropy factor measured by polarized beam for biological tissues

D. Fukutomi; K. Ishii; K. Awazu

doi:10.1117/12.2203194

9 December 2015 Determination of scattering coefficient considering wavelength and absorption dependence of anisotropy factor measured by polarized beam for biological tissues

D. Fukutomi, K. Ishii, K. Awazu

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Proceedings Volume 9792, Biophotonics Japan 2015; 97920T (2015) https://doi.org/10.1117/12.2203194
Event: SPIE/OSJ Biophotonics Japan, 2015, Tokyo, Japan

Abstract

Anisotropy factor g, one of the optical properties of biological tissues, is the most important parameter to accurately determine scattering coefficient μs in the inverse Monte Carlo (iMC) simulation. It has been reported that g has wavelength and absorption dependence, however, there are few attempts in order to calculate μs of biological tissue considering the wavelength and absorption dependence of g. In this study, the scattering angular distributions of biological tissue phantoms were measured in order to determine g by using goniometric measurements with three polarization conditions at strongly and weakly absorbing wavelengths of hemoglobin. Then, optical properties, especially, μs were measured by integrating sphere measurements and iMC simulation in order to confirm the influence of measured g on optical properties in comparison of with general value of g (0.9) for soft biological tissue. Consequently, it was found that μs was overestimated at strongly absorbing wavelength, however, μs was underestimated at weakly absorbing wavelength if the g was not considered its wavelength and absorption dependence.

Citation Download Citation

D. Fukutomi, K. Ishii, and K. Awazu "Determination of scattering coefficient considering wavelength and absorption dependence of anisotropy factor measured by polarized beam for biological tissues", Proc. SPIE 9792, Biophotonics Japan 2015, 97920T (9 December 2015); https://doi.org/10.1117/12.2203194

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KEYWORDS

Tissues

Absorption

Scattering

Polarization

Monte Carlo methods

Optical properties

Photons

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