Anisotropy factor g, one of the optical properties of biological tissues, is the most important parameter to accurately
determine scattering coefficient μs in the inverse Monte Carlo (iMC) simulation. It has been reported that g has
wavelength and absorption dependence, however, there are few attempts in order to calculate μs of biological tissue
considering the wavelength and absorption dependence of g. In this study, the scattering angular distributions of
biological tissue phantoms were measured in order to determine g by using goniometric measurements with three
polarization conditions at strongly and weakly absorbing wavelengths of hemoglobin. Then, optical properties,
especially, μs were measured by integrating sphere measurements and iMC simulation in order to confirm the influence
of measured g on optical properties in comparison of with general value of g (0.9) for soft biological tissue.
Consequently, it was found that μs was overestimated at strongly absorbing wavelength, however, μs was underestimated
at weakly absorbing wavelength if the g was not considered its wavelength and absorption dependence.