Quantitative phase imaging of cell division in yeast cells and E.coli using digital holographic microscopy

Vimal Prabhu Pandiyan; Renu John

doi:10.1117/12.2207425

Translator Disclaimer

9 December 2015 Quantitative phase imaging of cell division in yeast cells and E.coli using digital holographic microscopy

Vimal Prabhu Pandiyan; Renu John

Author Affiliations +

Proceedings Volume 9792, Biophotonics Japan 2015; 97920Y (2015) https://doi.org/10.1117/12.2207425
Event: SPIE/OSJ Biophotonics Japan, 2015, Tokyo, Japan

Abstract

Digital holographic microscope (DHM) is an emerging quantitative phase imaging technique with unique imaging scales and resolutions leading to multitude of applications. DHM is promising as a novel investigational and applied tool for cell imaging, studying the morphology and real time dynamics of cells and a number of related applications. The use of numerical propagation and computational digital optics offer unique flexibility to tune the depth of focus, and compensate for image aberrations. In this work, we report imaging the dynamics of cell division in E.coli and yeast cells using a DHM platform. We demonstrate 3-D and depth imaging as well as reconstruction of phase profiles of E.coli and yeast cells using the system. We record a digital hologram of E.coli and yeast cells and reconstruct the image using Fresnel propagation algorithm. We also use aberration compensation algorithms for correcting the aberrations that are introduced by the microscope objective in the object path using linear least square fitting techniques. This work demonstrates the strong potential of a DHM platform in 3-D live cell imaging, fast clinical quantifications and pathological applications.

Citation Download Citation

Vimal Prabhu Pandiyan and Renu John "Quantitative phase imaging of cell division in yeast cells and E.coli using digital holographic microscopy", Proc. SPIE 9792, Biophotonics Japan 2015, 97920Y (9 December 2015); https://doi.org/10.1117/12.2207425