16 April 2016 Single molecule force spectroscopy study of γ-polyglutamic acid by using atomic force microscopy
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Abstract
γ-PGA is a versatile multi-functional biopolymer possessing much useful biological functionality. Each biological function is associated with specific properties related to various forms of γ-PGA. However, to date, knowledge about the molecular mechanism to control specific structures of γ-PGA under a certain environmental conditions are unknown. In this study, we used single molecule force spectroscopy (SMFS) method to characterize the structure of γ-PGA in two environmental conditions. A water environment or 0.5 M MgCl2 salt solution was used to observe the effect of metal ion on γ-PGA structure at room temperature. The obtained results revealed that γ-PGA exists branched and/or cross-linked structure of γ-PGA in water or MgCl2 salt solution.
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Parbhej Ahamed, Parbhej Ahamed, Masaru Kawakami, Masaru Kawakami, Hidemitsu Furukawa, Hidemitsu Furukawa, } "Single molecule force spectroscopy study of γ-polyglutamic acid by using atomic force microscopy", Proc. SPIE 9802, Nanosensors, Biosensors, and Info-Tech Sensors and Systems 2016, 98020O (16 April 2016); doi: 10.1117/12.2218040; https://doi.org/10.1117/12.2218040
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