Adaptive segmentation of nuclei in H and E stained tendon microscopy

Bo-I Chuang; Po-Ting Wu; Jian-Han Hsu; I-Ming Jou; Fong-Chin Su; Yung-Nien Sun

doi:10.1117/12.2228807

9 December 2015 Adaptive segmentation of nuclei in H&S stained tendon microscopy

Bo-I Chuang; Po-Ting Wu; Jian-Han Hsu; I-Ming Jou; Fong-Chin Su; Yung-Nien Sun

Author Affiliations +

Proceedings Volume 9817, Seventh International Conference on Graphic and Image Processing (ICGIP 2015); 98170L (2015) https://doi.org/10.1117/12.2228807
Event: Seventh International Conference on Graphic and Image Processing, 2015, Singapore, Singapore

Abstract

Tendiopathy is a popular clinical issue in recent years. In most cases like trigger finger or tennis elbow, the pathology change can be observed under H and E stained tendon microscopy. However, the qualitative analysis is too subjective and thus the results heavily depend on the observers. We develop an automatic segmentation procedure which segments and counts the nuclei in H and E stained tendon microscopy fast and precisely. This procedure first determines the complexity of images and then segments the nuclei from the image. For the complex images, the proposed method adopts sampling-based thresholding to segment the nuclei. While for the simple images, the Laplacian-based thresholding is employed to re-segment the nuclei more accurately. In the experiments, the proposed method is compared with the experts outlined results. The nuclei number of proposed method is closed to the experts counted, and the processing time of proposed method is much faster than the experts’.

Citation Download Citation

Bo-I Chuang, Bo-I Chuang, Po-Ting Wu, Po-Ting Wu, Jian-Han Hsu, Jian-Han Hsu, I-Ming Jou, I-Ming Jou, Fong-Chin Su, Fong-Chin Su, Yung-Nien Sun, Yung-Nien Sun, } "Adaptive segmentation of nuclei in H&S stained tendon microscopy", Proc. SPIE 9817, Seventh International Conference on Graphic and Image Processing (ICGIP 2015), 98170L (9 December 2015); doi: 10.1117/12.2228807; https://doi.org/10.1117/12.2228807

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $14.40

Non-members: $18.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years