Adaptive segmentation of nuclei in H and E stained tendon microscopy

Bo-I Chuang; Po-Ting Wu; Jian-Han Hsu; I-Ming Jou; Fong-Chin Su; Yung-Nien Sun

doi:10.1117/12.2228807

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9 December 2015 Adaptive segmentation of nuclei in H&S stained tendon microscopy

Bo-I Chuang, Po-Ting Wu, Jian-Han Hsu, I-Ming Jou, Fong-Chin Su, Yung-Nien Sun

Proceedings Volume 9817, Seventh International Conference on Graphic and Image Processing (ICGIP 2015); 98170L (2015) https://doi.org/10.1117/12.2228807
Event: Seventh International Conference on Graphic and Image Processing, 2015, Singapore, Singapore

Abstract

Tendiopathy is a popular clinical issue in recent years. In most cases like trigger finger or tennis elbow, the pathology change can be observed under H and E stained tendon microscopy. However, the qualitative analysis is too subjective and thus the results heavily depend on the observers. We develop an automatic segmentation procedure which segments and counts the nuclei in H and E stained tendon microscopy fast and precisely. This procedure first determines the complexity of images and then segments the nuclei from the image. For the complex images, the proposed method adopts sampling-based thresholding to segment the nuclei. While for the simple images, the Laplacian-based thresholding is employed to re-segment the nuclei more accurately. In the experiments, the proposed method is compared with the experts outlined results. The nuclei number of proposed method is closed to the experts counted, and the processing time of proposed method is much faster than the experts’.

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Bo-I Chuang, Po-Ting Wu, Jian-Han Hsu, I-Ming Jou, Fong-Chin Su, and Yung-Nien Sun "Adaptive segmentation of nuclei in H&S stained tendon microscopy", Proc. SPIE 9817, Seventh International Conference on Graphic and Image Processing (ICGIP 2015), 98170L (9 December 2015); https://doi.org/10.1117/12.2228807

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