5 May 2016 Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy
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Time-correlated single photon counting (TCSPC) is a widely used, sensitive, precise, robust and mature technique to measure photon arrival times in applications such as fluorescence spectroscopy and microscopy, light detection and ranging (lidar) and optical tomography. Wide-field TCSPC detection techniques, where the position and the arrival time of the photons are recorded simultaneously, have seen several advances in the last few years, from the microsecond to the picosecond time scale. Here, we summarise some of our recent work in this field with emphasis on microsecond resolution phosphorescence lifetime imaging (PLIM) and nanosecond fluorescence lifetime imaging (FLIM) microscopy.
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Klaus Suhling, Klaus Suhling, Liisa M. Hirvonen, Liisa M. Hirvonen, Wolfgang Becker, Wolfgang Becker, Stefan Smietana, Stefan Smietana, Holger Netz, Holger Netz, James Milnes, James Milnes, Thomas Conneely, Thomas Conneely, Alix Le Marois, Alix Le Marois, Ottmar Jagutzki, Ottmar Jagutzki, } "Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy", Proc. SPIE 9858, Advanced Photon Counting Techniques X, 98580J (5 May 2016); doi: 10.1117/12.2227198; https://doi.org/10.1117/12.2227198


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