17 May 2016 Active fluid mixing with magnetic microactuators for capture of salmonella
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Abstract
Detection of low concentrations of bacteria in food samples is a challenging process. Key to this process is the separation of the target from the food matrix. We demonstrate magnetic beads and magnetic micro-cilia based microfluidic mixing and capture, which are particularly useful for pre-concentrating the target. The first method we demonstrate makes use of magnetic microbeads held on to NiFe discs on the surface of the substrate. These beads are rotated around the magnetic discs by rotating the external magnetic field. The second method we demonstrate shows the use of cilia which extends into the fluid and is manipulated by a rotating external field. Magnetic micro-features were fabricated by evaporating NiFe alloy at room temperature, on to patterned photoresist. The high magnetic permeability of NiFe allows for maximum magnetic force on the features. The magnetic features were actuated using an external rotating magnet up to frequencies of 50Hz. We demonstrate active mixing produced by the microbeads and the cilia in a microchannel. Also, we demonstrate the capture of target species in a sample using microbeads.
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S. Hanasoge, S. Hanasoge, D. Owen, D. Owen, M. Ballard, M. Ballard, Z. Mills, Z. Mills, J. Xu, J. Xu, M. Erickson, M. Erickson, P. J. Hesketh, P. J. Hesketh, A. Alexeev, A. Alexeev, } "Active fluid mixing with magnetic microactuators for capture of salmonella", Proc. SPIE 9864, Sensing for Agriculture and Food Quality and Safety VIII, 986405 (17 May 2016); doi: 10.1117/12.2225571; https://doi.org/10.1117/12.2225571
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