Resourcesat-2A is a follow-on mission of Resourcesat-2, belongs to Indian Remote Sensing Program. It is expected to be launched in 2016 and is dedicated mainly to agricultural applications. One of the payloads, LISS3* is a medium resolution (23.5 m) sensor having four multispectral bands from 450 to 1650 nm. These spectral bands are named as B2 (550 nm), B3 (650 nm), B4 (815 nm) and B5 (1625 nm) respectively covering Visible, Near Infrared (NIR) and Short Wave Infrared (SWIR) regions. In order to provide quality data to the user community for long term scientific applications pre-flight ground calibration is carried out. This paper describes pre-flight spectral and radiometric calibration of LISS3* payload and its performance evaluation. Since it is a continuity mission to Resourcesat-2, which was launched in April 2011 so for generating long-term data record and correlation with previous observations, its parameters are compared with Resourcesat-2 LISS3* payload. The main spectral parameters like central wavelength, and pass band is determined using system level spectral response and compared for both the mission and differences are outlined. The next important exercise is pre-flight radiometric calibration, which was carried out in laboratory using a standard integrating sphere traceable to NIST standards. This paper highlights the technique adopted during pre-flight calibration of the radiometric response and performance assessment of all 4 bands of LISS3* in terms of major electro-optical parameters like Signal to Noise Ratio (SNR), Saturation Radiance (SR) etc. The observed SR shows that the sensor can measure spectral radiance from Earth up to 100% albedo.