Development of cadmium-free quantum dot for intracellular labelling through electroporation or lipid-calcium-phosphate

Ying-Feng Liu; Wei-Ling Hung; Tzh-Yin Hou; Hsiu-Ying Huang; Cheng-An J. Lin

doi:10.1117/12.2225864

19 April 2016 Development of cadmium-free quantum dot for intracellular labelling through electroporation or lipid-calcium-phosphate

Ying-Feng Liu, Wei-Ling Hung, Tzh-Yin Hou, Hsiu-Ying Huang, Cheng-An J. Lin

Author Affiliations +

Proceedings Volume 9884, Nanophotonics VI; 988428 (2016) https://doi.org/10.1117/12.2225864
Event: SPIE Photonics Europe, 2016, Brussels, Belgium

Abstract

Traditional fluorescent labelling techniques has severe photo-bleaching problem such as organic dyes and fluorescent protein. Quantum dots made up of traditional semiconductor (CdSe/ZnS) material has sort of biological toxicity. This research has developed novel Cd-free quantum dots divided into semiconductor (Indium phosphide, InP) and noble metal (Gold). Former has lower toxicity compared to traditional quantum dots. Latter consisting of gold (III) chloride (AuCl3) and toluene utilizes sonochemical preparation and different stimulus to regulate fluorescent wavelength. Amphoteric macromolecule surface technology and ligand Exchange in self-Assembled are involved to develop hydrophilic nanomaterials which can regulate the number of grafts per molecule of surface functional groups. Calcium phosphate (CaP) nanoparticle (NP) with an asymmetric lipid bilayer coating technology developed for intracellular delivery and labelling has synthesized Cd-free quantum dots possessing high brightness and multi-fluorescence successfully. Then, polymer coating and ligand exchange transfer to water-soluble materials to produce liposome nanomaterials as fluorescent probes and enhancing medical applications of nanotechnology.

Citation Download Citation

Ying-Feng Liu, Ying-Feng Liu, Wei-Ling Hung, Wei-Ling Hung, Tzh-Yin Hou, Tzh-Yin Hou, Hsiu-Ying Huang, Hsiu-Ying Huang, Cheng-An J. Lin, Cheng-An J. Lin, "Development of cadmium-free quantum dot for intracellular labelling through electroporation or lipid-calcium-phosphate", Proc. SPIE 9884, Nanophotonics VI, 988428 (19 April 2016); doi: 10.1117/12.2225864; https://doi.org/10.1117/12.2225864

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $14.40

Non-members: $18.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years