27 April 2016 Towards field malaria diagnosis using surface enhanced Raman spectroscopy
Author Affiliations +
Abstract
We report three strategies of surface enhanced Raman spectroscopy (SERS) for β-hematin and hemozoin detection in malaria infected human blood, which can be potentially developed for field malaria diagnosis. In the first strategy, we used silver coated magnetic nanoparticles (Fe3O4@Ag) in combination with an external magnetic field to enhance the Raman signal of β-hematin. Then we developed two SERS methods without the requirement of magnetic field for malaria infection diagnosis. In Method 1, silver nanoparticles were synthesized separately and then mixed with lysed blood just like in traditional SERS measurements; while in Method 2, we developed an ultrasensitive SERS method by synthesizing silver nanoparticles directly inside the parasites of Plasmodium falciparum. Method 2 can be also used to detect single parasites in the ring stage.
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Keren Chen, Aoli Xiong, Clement Yuen, Peter Preiser, Quan Liu, "Towards field malaria diagnosis using surface enhanced Raman spectroscopy", Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 98870M (27 April 2016); doi: 10.1117/12.2227042; https://doi.org/10.1117/12.2227042
PROCEEDINGS
8 PAGES


SHARE
Back to Top