27 April 2016 Towards field malaria diagnosis using surface enhanced Raman spectroscopy
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We report three strategies of surface enhanced Raman spectroscopy (SERS) for β-hematin and hemozoin detection in malaria infected human blood, which can be potentially developed for field malaria diagnosis. In the first strategy, we used silver coated magnetic nanoparticles (Fe3O4@Ag) in combination with an external magnetic field to enhance the Raman signal of β-hematin. Then we developed two SERS methods without the requirement of magnetic field for malaria infection diagnosis. In Method 1, silver nanoparticles were synthesized separately and then mixed with lysed blood just like in traditional SERS measurements; while in Method 2, we developed an ultrasensitive SERS method by synthesizing silver nanoparticles directly inside the parasites of Plasmodium falciparum. Method 2 can be also used to detect single parasites in the ring stage.
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Keren Chen, Keren Chen, Aoli Xiong, Aoli Xiong, Clement Yuen, Clement Yuen, Peter Preiser, Peter Preiser, Quan Liu, Quan Liu, "Towards field malaria diagnosis using surface enhanced Raman spectroscopy", Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 98870M (27 April 2016); doi: 10.1117/12.2227042; https://doi.org/10.1117/12.2227042

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