27 April 2016 Hybrid nanosensor for colorimetric and ultrasensitive detection of nuclease contaminations
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Abstract
Nucleases are ubiquitous enzymes that degrade DNA or RNA, thus they can prejudice the good outcome of molecular biology experiments involving nucleic acids. We propose a colorimetric test for the naked-eye detection of nuclease contaminations. The system uses an hybrid nanosensor, based on gold nanoparticles functionalized with DNA probes. Our assay is rapid, instrument-free, simple and low-cost. Moreover, it reaches sensitivity equal or better than those of commercial kits, and presents a lot of advantageous aspects. Therefore, it is very competitive, with a real market potential. This test will be relevant in routine process monitoring in scientific laboratories, and in quality control in clinical laboratories and industrial processes, allowing the simultaneous detection of nucleases with different substrate specificities and large-scale screening.
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Paola Cecere, Paola Cecere, Paola Valentini, Paola Valentini, Pier Paolo Pompa, Pier Paolo Pompa, "Hybrid nanosensor for colorimetric and ultrasensitive detection of nuclease contaminations", Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 98870O (27 April 2016); doi: 10.1117/12.2225929; https://doi.org/10.1117/12.2225929
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