27 April 2016 A gold nanoparticles-based colorimetric test to detect single nucleotide polymorphisms for improvement of personalized therapy of psoriasis
Author Affiliations +
Abstract
We report a simple, rapid and low-cost test, based on gold nanoparticles, for the naked-eye colorimetric detection of a signature of single nucleotide polymorphisms (SNPs) relevant for the personalized medicine of psoriasis patients. We validated the colorimetric assay on real-world DNA samples from a cohort of 30 psoriasis patients and we compared the results, in double-blind, with those obtained with two state-of-the-art instrumental techniques, namely reverse dot blotting and direct sequencing, finding 100% agreement. We demonstrated high accuracy, sensitivity and specificity of the colorimetric test that can be easily adapted for the genotypization of different SNPs, important for the pharmacogenomics of various diseases, and in other fields, such as food traceability and population structure analysis.
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Alessandra Marsella, Alessandra Marsella, Paola Valentini, Paola Valentini, Paolo Tarantino, Paolo Tarantino, Maurizio Congedo, Maurizio Congedo, Pier Paolo Pompa, Pier Paolo Pompa, } "A gold nanoparticles-based colorimetric test to detect single nucleotide polymorphisms for improvement of personalized therapy of psoriasis", Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 98870P (27 April 2016); doi: 10.1117/12.2225930; https://doi.org/10.1117/12.2225930
PROCEEDINGS
10 PAGES


SHARE
Back to Top