Analysis of energy metabolism of HeLa cancer cells in vitro and in vivo using fluorescence lifetime microscopy

Maria Lukina; Marina Shirmanova; Varvara Dudenkova; Irina Druzhkova; Anastasia Shumilova; Elena Zagaynova

doi:10.1117/12.2227488

27 April 2016 Analysis of energy metabolism of HeLa cancer cells in vitro and in vivo using fluorescence lifetime microscopy

Maria Lukina, Marina Shirmanova, Varvara Dudenkova, Irina Druzhkova, Anastasia Shumilova, Elena Zagaynova

Author Affiliations +

Proceedings Volume 9887, Biophotonics: Photonic Solutions for Better Health Care V; 98872S (2016) https://doi.org/10.1117/12.2227488
Event: SPIE Photonics Europe, 2016, Brussels, Belgium

Abstract

The aim of the present work was to study energy metabolism in human cervical carcinoma (HeLa) cells in vitro and in vivo using two-photon FLIM. Cellular metabolism was examined by monitoring of the fluorescence lifetimes of free and protein-bound forms of NAD(P)H and FAD and their relative contributions. Two-photon fluorescence and second harmonic generation microscopy as well as standard histopathology with hematoxylin and eosin were used to characterize tissue structure. Cellular metabolism was analyzed in cancer cells co-cultured with human fibroblasts and in tumor xenografts transplanted to nude mice. In the HeLa-huFB co-culture we observed a metabolic shift from OXPHOS toward glycolysis in cancer cells, and from glycolysis to OXPHOS in fibroblasts, starting from Day 2 of co-culturing. In the tumor tissue we detected metabolic heterogeneity with more glycolytic metabolism of cancer cells in the stroma-rich zones. The results of the study are of a great importance for understanding metabolic behavior of tumors and for development of anticancer drugs targeted to metabolic pathways.

Citation Download Citation

Maria Lukina, Marina Shirmanova, Varvara Dudenkova, Irina Druzhkova, Anastasia Shumilova, and Elena Zagaynova "Analysis of energy metabolism of HeLa cancer cells in vitro and in vivo using fluorescence lifetime microscopy", Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 98872S (27 April 2016); https://doi.org/10.1117/12.2227488

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Cancer

Tumors

Mode conditioning cables

Luminescence

Fluorescence lifetime imaging

In vivo imaging

In vitro testing

Show All Keywords

Keywords/Phrases

Search In:

Publication Years