27 April 2016 Analysis of energy metabolism of HeLa cancer cells in vitro and in vivo using fluorescence lifetime microscopy
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Abstract
The aim of the present work was to study energy metabolism in human cervical carcinoma (HeLa) cells in vitro and in vivo using two-photon FLIM. Cellular metabolism was examined by monitoring of the fluorescence lifetimes of free and protein-bound forms of NAD(P)H and FAD and their relative contributions. Two-photon fluorescence and second harmonic generation microscopy as well as standard histopathology with hematoxylin and eosin were used to characterize tissue structure. Cellular metabolism was analyzed in cancer cells co-cultured with human fibroblasts and in tumor xenografts transplanted to nude mice. In the HeLa-huFB co-culture we observed a metabolic shift from OXPHOS toward glycolysis in cancer cells, and from glycolysis to OXPHOS in fibroblasts, starting from Day 2 of co-culturing. In the tumor tissue we detected metabolic heterogeneity with more glycolytic metabolism of cancer cells in the stroma-rich zones. The results of the study are of a great importance for understanding metabolic behavior of tumors and for development of anticancer drugs targeted to metabolic pathways.
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Maria Lukina, Maria Lukina, Marina Shirmanova, Marina Shirmanova, Varvara Dudenkova, Varvara Dudenkova, Irina Druzhkova, Irina Druzhkova, Anastasia Shumilova, Anastasia Shumilova, Elena Zagaynova, Elena Zagaynova, } "Analysis of energy metabolism of HeLa cancer cells in vitro and in vivo using fluorescence lifetime microscopy", Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 98872S (27 April 2016); doi: 10.1117/12.2227488; https://doi.org/10.1117/12.2227488
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