27 April 2016 Analysis of energy metabolism of HeLa cancer cells in vitro and in vivo using fluorescence lifetime microscopy
Author Affiliations +
The aim of the present work was to study energy metabolism in human cervical carcinoma (HeLa) cells in vitro and in vivo using two-photon FLIM. Cellular metabolism was examined by monitoring of the fluorescence lifetimes of free and protein-bound forms of NAD(P)H and FAD and their relative contributions. Two-photon fluorescence and second harmonic generation microscopy as well as standard histopathology with hematoxylin and eosin were used to characterize tissue structure. Cellular metabolism was analyzed in cancer cells co-cultured with human fibroblasts and in tumor xenografts transplanted to nude mice. In the HeLa-huFB co-culture we observed a metabolic shift from OXPHOS toward glycolysis in cancer cells, and from glycolysis to OXPHOS in fibroblasts, starting from Day 2 of co-culturing. In the tumor tissue we detected metabolic heterogeneity with more glycolytic metabolism of cancer cells in the stroma-rich zones. The results of the study are of a great importance for understanding metabolic behavior of tumors and for development of anticancer drugs targeted to metabolic pathways.
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Maria Lukina, Maria Lukina, Marina Shirmanova, Marina Shirmanova, Varvara Dudenkova, Varvara Dudenkova, Irina Druzhkova, Irina Druzhkova, Anastasia Shumilova, Anastasia Shumilova, Elena Zagaynova, Elena Zagaynova, } "Analysis of energy metabolism of HeLa cancer cells in vitro and in vivo using fluorescence lifetime microscopy", Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 98872S (27 April 2016); doi: 10.1117/12.2227488; https://doi.org/10.1117/12.2227488

Back to Top