27 April 2016 Application of fluorescent dyes for some problems of bioelectromagnetics
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Fluorescent organic dyes solutions are used for non-contact measurement of the millimeter wave absorption in liquids simulating biological tissue. There is still not any certain idea of the physical mechanism describing this process despite the widespread technology of microwave radiation in the food industry, biotechnology and medicine. For creating adequate physical model one requires an accurate command of knowledge concerning to the relation between millimeter waves and irradiated object. There were three H-bonded liquids selected as the samples with different coefficients of absorption in the millimeter range like water (strong absorption), glycerol (medium absorption) and ethylene glycol (light absorption). The measurements showed that the greatest response to the action of microwaves occurs for glycerol solutions: R6G (building-up luminescence) and RC (fading luminescence). For aqueous solutions the signal is lower due to lower quantum efficiency of luminescence, and for ethylene glycol — due to the low absorption of microwaves. In the area of exposure a local increase of temperature was estimated. For aqueous solutions of both dyes the maximum temperature increase is about 7° C caused with millimeter waves absorption, which coincides with the direct radio physical measurements and confirmed by theoretical calculations. However, for glycerol solution R6G temperature equivalent for building-up luminescence is around 9° C, and for the solution of ethylene glycol it’s about 15°. It is assumed the possibility of non-thermal effect of microwaves on the different processes and substances. The application of this non-contact temperature sensing is a simple and novel method to detect temperature change in small biological objects.
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Danylo Babich, Danylo Babich, Alexandr Kylsky, Alexandr Kylsky, Valentina Pobiedina, Valentina Pobiedina, Andrey Yakunov, Andrey Yakunov, "Application of fluorescent dyes for some problems of bioelectromagnetics", Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 988735 (27 April 2016); doi: 10.1117/12.2227373; https://doi.org/10.1117/12.2227373

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