27 April 2016 Comparison of Raman spectroscopy equipment for tissues and biofluids analysis
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Abstract
In this study we demonstrate a comparative analysis of blood serum and normal human skin by Raman spectroscopy with application of different spectroscopic equipment. For serum analysis we measure a total concentration of proteins and compared it with intensity of 1002 cm-1 Raman peak. Standard deviation for protein control in blood serum differed from 7.4% to 19% for different spectroscopic setups. For human skin control we used three Raman peaks near 1340, 1450 and 1650 cm-1. Measurements of different skin samples were analyzed on the phase plane to find areas corresponding to the normal skin. Taking into account the different sensitivities of the detected signal with different detectors in the spectral range 810-950 nm we calculated correction coefficients allowed for making comparison of spectral measurements made on different spectrometers with ranging not exceeding 21%.
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Yu. A. Khristoforova, Yu. A. Khristoforova, I. A. Bratchenko, I. A. Bratchenko, O. O. Myakinin, O. O. Myakinin, D. N. Artemyev, D. N. Artemyev, V. P. Zakharov, V. P. Zakharov, "Comparison of Raman spectroscopy equipment for tissues and biofluids analysis", Proc. SPIE 9887, Biophotonics: Photonic Solutions for Better Health Care V, 98873E (27 April 2016); doi: 10.1117/12.2227878; https://doi.org/10.1117/12.2227878
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