29 April 2016 Biosensors based on Si3N4 asymmetric Mach-Zehnder interferometers
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Abstract
In this work, we present a study on photonic biosensors based on Si3N4 asymmetric Mach-Zehnder Interferometers (aMZI) for Aflatoxin M1 (AFM1) detection. AFM1 is an hepatotoxic and a carcinogenic toxin present in milk. The biosensor is based on an array of four Si3N4 aMZI that are optimized for 850nm wavelength. We measure the bulk Sensitivity (S) and the Limit of Detection (LOD) of our devices. In the array, three devices are exposed and have very similar sensitivities. The fourth aMZI, which is covered by SiO2, is used as an internal reference for laser (a VCSEL) and temperature fluctuations. We measured a phase sensitivity of 14300±400 rad/RIU. To characterize the LOD of the sensors, we measure the uncertainty of the experimental readout system. From the measurements on three aMZI, we observe the same value of LOD, which is ≈ 4.5×10−7 RIU. After the sensor characterization on homogeneous sensing, we test the surface sensing performances by flowing specific Aflatoxin M1 and non-specific Ochratoxin in 50 mM MES pH 6.6 buffer on the top of the sensors functionalized with Antigen-Recognising Fragments (Fab’). The difference between specific and non-specific signals shows the specificity of our sensors. A moderate regeneration of the sensors is obtained by using glycine solution.
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Tatevik Chalyan, Tatevik Chalyan, Laura Pasquardini, Laura Pasquardini, Floris Falke, Floris Falke, Manuela Zanetti, Manuela Zanetti, Romain Guider, Romain Guider, Davide Gandolfi, Davide Gandolfi, Eric Schreuder, Eric Schreuder, Cecilia Pederzolli, Cecilia Pederzolli, René G. Heideman, René G. Heideman, Lorenzo Pavesi, Lorenzo Pavesi, } "Biosensors based on Si3N4 asymmetric Mach-Zehnder interferometers", Proc. SPIE 9899, Optical Sensing and Detection IV, 98991S (29 April 2016); doi: 10.1117/12.2227695; https://doi.org/10.1117/12.2227695
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