Biosensors based on Si3N4 asymmetric Mach-Zehnder interferometers

Tatevik Chalyan; Laura Pasquardini; Floris Falke; Manuela Zanetti; Romain Guider; Davide Gandolfi; Eric Schreuder; Cecilia Pederzolli; René G. Heideman; Lorenzo Pavesi

doi:10.1117/12.2227695

29 April 2016 Biosensors based on Si₃N₄ asymmetric Mach-Zehnder interferometers

Tatevik Chalyan, Laura Pasquardini, Floris Falke, Manuela Zanetti, Romain Guider, Davide Gandolfi, Eric Schreuder, Cecilia Pederzolli, René G. Heideman, Lorenzo Pavesi

Author Affiliations +

Proceedings Volume 9899, Optical Sensing and Detection IV; 98991S (2016) https://doi.org/10.1117/12.2227695
Event: SPIE Photonics Europe, 2016, Brussels, Belgium

Abstract

In this work, we present a study on photonic biosensors based on Si₃N₄ asymmetric Mach-Zehnder Interferometers (aMZI) for Aﬂatoxin M1 (AFM1) detection. AFM1 is an hepatotoxic and a carcinogenic toxin present in milk. The biosensor is based on an array of four Si₃N₄ aMZI that are optimized for 850nm wavelength. We measure the bulk Sensitivity (S) and the Limit of Detection (LOD) of our devices. In the array, three devices are exposed and have very similar sensitivities. The fourth aMZI, which is covered by SiO₂, is used as an internal reference for laser (a VCSEL) and temperature ﬂuctuations. We measured a phase sensitivity of 14300±400 rad/RIU. To characterize the LOD of the sensors, we measure the uncertainty of the experimental readout system. From the measurements on three aMZI, we observe the same value of LOD, which is ≈ 4.5×10⁻⁷ RIU. After the sensor characterization on homogeneous sensing, we test the surface sensing performances by ﬂowing speciﬁc Aﬂatoxin M1 and non-speciﬁc Ochratoxin in 50 mM MES pH 6.6 buﬀer on the top of the sensors functionalized with Antigen-Recognising Fragments (Fab’). The diﬀerence between speciﬁc and non-speciﬁc signals shows the speciﬁcity of our sensors. A moderate regeneration of the sensors is obtained by using glycine solution.

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Tatevik Chalyan, Laura Pasquardini, Floris Falke, Manuela Zanetti, Romain Guider, Davide Gandolfi, Eric Schreuder, Cecilia Pederzolli, René G. Heideman, and Lorenzo Pavesi "Biosensors based on Si₃N₄ asymmetric Mach-Zehnder interferometers", Proc. SPIE 9899, Optical Sensing and Detection IV, 98991S (29 April 2016); https://doi.org/10.1117/12.2227695

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