29 April 2016 Detection of bacteria using bacteriophage with hollow gold nanostructures immobilized fiber optic sensor
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Abstract
Hollow gold nanostructures (HGNS) have been used in variety of optical biosensors due to their inherent advantage of operating at near infra red (NIR) wavelength, large extinction coefficient and high dielectric sensitivity. The absorption wavelength of these nanostructures can be modulated by changing the ratio of hollow region to the core shell thickness. The aim of the present study is to incorporate the properties of HGNS, to develop LSPR based U-bent fiber optic sensor for detection of pathogens. The detection was carried out using an experimental set up consisting of a white light source, 200 μm diameter optical fiber having bend diameter of 1.6 mm ± 0. 2 mm and a spectrometer. The HGNS were immobilized on the decladded portion of the fiber optic probe by chemisorptions. The effective plasmon penetration depth of the HGNS immobilized fiber optic sensor was approximated by using alternating layers of positively and negatively charged polyelectrolytes. The HGNS immobilized U-bent fiber optic sensor was used for detection of E.coli B40 strain using bacteriophage T4. The preliminary experiments were carried out with 104 cfu/ml of E.coli B40 and the change in absorbance obtained was approx. 0.042 ± 0.0045 abs. units (n = 3). The response of this sensor was found to be better than spherical gold nanoparticle immobilized sensing platforms.
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Pallavi Halkare, Pallavi Halkare, Nirmal Punjabi, Nirmal Punjabi, Jigme Wangchuk, Jigme Wangchuk, Kiran Kondabagil, Kiran Kondabagil, Soumyo Mukherji, Soumyo Mukherji, } "Detection of bacteria using bacteriophage with hollow gold nanostructures immobilized fiber optic sensor", Proc. SPIE 9899, Optical Sensing and Detection IV, 98992Z (29 April 2016); doi: 10.1117/12.2230639; https://doi.org/10.1117/12.2230639
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