26 January 2016 Study on fluorescence spectra of thiamine, riboflavin and pyridoxine
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Proceedings Volume 9903, Seventh International Symposium on Precision Mechanical Measurements; 99030H (2016) https://doi.org/10.1117/12.2211248
Event: Seventh International Symposium on Precision Mechanical Measurements, 2015, Xia'men, China
Abstract
This paper presents the intrinsic fluorescence characteristics of vitamin B1, B2 and B6 measured with 3D fluorescence Spectrophotometer. Three strong fluorescence areas of vitamin B2 locate at λex/λem=270/525nm, 370/525nm and 450/525nm, one fluorescence areas of vitamin B1 locates at λex/λem=370/460nm, two fluorescence areas of vitamin B6 locate at λex/λem=250/370nm and 325/370nm were found. The influence of pH of solution to the fluorescence profile was also discussed. Using the PARAFAC algorithm, 10 vitamin B1, B2 and B6 mixed solutions were successfully decomposed, and the emission profiles, excitation profiles, central wavelengths and the concentration of the three components were retrieved precisely through about 5 iteration times.
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Hui Yang, Hui Yang, Xue Xiao, Xue Xiao, Xuesong Zhao, Xuesong Zhao, Lan Hu, Lan Hu, Caofang Lv, Caofang Lv, Zhangkun Yin, Zhangkun Yin, } "Study on fluorescence spectra of thiamine, riboflavin and pyridoxine", Proc. SPIE 9903, Seventh International Symposium on Precision Mechanical Measurements, 99030H (26 January 2016); doi: 10.1117/12.2211248; https://doi.org/10.1117/12.2211248
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