21 April 2016 The effect of housing temperature on the growth of CT26 tumor expressing fluorescent protein EGFP
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Abstract
To date, the effect of housing temperature on tumor development in the immunocompetent mice has been studied on poorly immunogenic cancer models. Standard housing temperature 20–26°C was shown to cause chronic metabolic cold stress and promote tumor progression via suppression of the antitumor immune response, whereas a thermoneutral temperature 30-31°C was more preferable for normal metabolism of mice and inhibited tumor growth. Our work represents the first attempt to discover the potential effect of housing temperature on the development of highly immunogenic tumor. EGFP-expressing murine colon carcinoma CT26 generated in Balb/c mice was used as a tumor model. No statistically significant differences were shown in tumor incidences and growth rates at 20°С, 25°С and 30°С for non-modified CT26. Maintaining mice challenged with CT26-EGFP cells at 30°С led to complete inhibition of tumor development. In summary, we demonstrated that the housing temperature is important for the regulation of growth of highly immunogenic tumors in mice through antitumor immunity.
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Diana V. Yuzhakova, Marina V. Shirmanova, Irina V. Lapkina, Ekaterina O. Serebrovskaya, Sergey A. Lukyanov, Elena V. Zagaynova, "The effect of housing temperature on the growth of CT26 tumor expressing fluorescent protein EGFP", Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 991709 (21 April 2016); doi: 10.1117/12.2228524; https://doi.org/10.1117/12.2228524
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