21 April 2016 Quantitative measurement of blood flow dynamics in chorioallantoic membrane of chicken embryo using laser Doppler anemometry
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Abstract
We report the results on in ovo application of developed Laser Doppler Anemometer (LDA) device. The chorioallantoic membrane (CAM) of 9-13 days chicken embryos was used as a biological model that allows an easy access to both arterial and venous vessels of different size. The key point of our study was to find out how the periodic and aperiodic pulsations of blood flow (which are inevitable in living organism) will affect the LDA functions and measuring capability. Specifically, we (i) developed the technique to extract and refine the pulse rhythm from the signal received from a vessel, and (ii) analyzed the changes in power spectra of LDA signal that are caused by heart beating and considerably complicate the reliable measurement of Doppler shift. Our main conclusion is that the algorithm of LDA data processing need to be improved, and this possibly can be done by counting the information on current phase of cardiac cycle.
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M. A. Borozdova, M. A. Borozdova, E. S. Stiukhina, E. S. Stiukhina, A. A. Sdobnov, A. A. Sdobnov, I. V. Fedosov, I. V. Fedosov, D. E. Postnov, D. E. Postnov, V. V. Tuchin, V. V. Tuchin, } "Quantitative measurement of blood flow dynamics in chorioallantoic membrane of chicken embryo using laser Doppler anemometry", Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 99170W (21 April 2016); doi: 10.1117/12.2229830; https://doi.org/10.1117/12.2229830
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