Detection of sulfonamide drug in urine using liquid-liquid extraction and surface-enhanced Raman spectroscopy

Natalia E. Markina; Victoria V. Shalabay; Andrey M. Zakharevich; Alexey V. Markin

doi:10.1117/12.2228866

21 April 2016 Detection of sulfonamide drug in urine using liquid-liquid extraction and surface-enhanced Raman spectroscopy

Natalia E. Markina, Victoria V. Shalabay, Andrey M. Zakharevich, Alexey V. Markin

Author Affiliations +

Proceedings Volume 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS); 99170X (2016) https://doi.org/10.1117/12.2228866
Event: Saratov Fall Meeting 2015, 2015, Saratov, Russian Federation

Abstract

In this article we have applied liquid-liquid extraction (LLE) as a sample preparation technique for detection of sulfadimethoxine (one of sulfonamide drugs) in urine using surface-enhanced Raman spectroscopy (SERS). SERS substrate based on silver nanoparticles has been prepared by citrate reduction of silver nitrate. Obtained calibration curve (SERS intensity vs. sulfadimethoxine concentration) has been used for detection of sulfadimethoxine in human urine samples artificially contaminated by sulfadimethoxine. Three different solvents (ethyl acetate, diethyl ether, chloroform) have been used for LLE performance tests. Chloroform being found as the most effective one based on calculation of recoveries after SERS measurements. Thus we would like to propose fast (less than 20 minutes), simple and sensitive (detection limit up to 1 μg/ml) test for detecting sulfa drugs in urine using a combination of SERS with LLE with sample volume as low as 100 μL. Such test can be applied for evaluation of the degree of drug extraction from human body and half-life of such drug applied in the course of therapeutic treatments of certain diseases.

Citation Download Citation

Natalia E. Markina, Victoria V. Shalabay, Andrey M. Zakharevich, and Alexey V. Markin "Detection of sulfonamide drug in urine using liquid-liquid extraction and surface-enhanced Raman spectroscopy", Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 99170X (21 April 2016); https://doi.org/10.1117/12.2228866

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available