21 April 2016 Investigation of cell proliferative activity on the surface of the nanocomposite material produced by laser radiation
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Abstract
A new method for the formation of composite nanomaterials based on multi-walled and single-walled carbon nanotubes (CNT) on a silicon substrate has been developed. Formation is carried out by ultrasound coating of a silicon substrate by homogenous dispersion of CNTs in the albumin matrix and further irradiation with the continuous laser beam with a wavelength of 810 nm and power of 5.5 watts. The high electrical conductivity of CNTs provides its structuring under the influence of the laser radiation electric field. The result is a scaffold that provides high mechanical strength of nanocomposite material (250 MPa). For in vitro studies of materials biocompatibility a method of cell growth microscopic analysis was developed. Human embryonic fibroblasts (EPP) were used as biological cells. Investigation of the interaction between nanocomposite material and cells was carried out by optical and atomic force microscopy depending on the time of cells incubation. The study showed that after 3 hours incubation EPP were fixed on the substrate surface, avoiding the surface of the composite material. However, after 24 hours of incubation EPP fix on the sample surface and then begin to grow and divide. After 72 hours of incubation, the cells completely fill the sample surface of nanocomposite material. Thus, a nanocomposite material based on CNTs in albumin matrix does not inhibit cell growth on its surface, and favours their growth. The nanocomposite material can be used for creating soft tissue implants
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N. N. Zhurbina, N. N. Zhurbina, U. E. Kurilova, U. E. Kurilova, L. P. Ickitidze, L. P. Ickitidze, V. M. Podgaetsky, V. M. Podgaetsky, S. V. Selishchev, S. V. Selishchev, I. A. Suetina, I. A. Suetina, M. V. Mezentseva, M. V. Mezentseva, E. M. Eganova, E. M. Eganova, A. A. Pavlov, A. A. Pavlov, A. Yu. Gerasimenko, A. Yu. Gerasimenko, } "Investigation of cell proliferative activity on the surface of the nanocomposite material produced by laser radiation", Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 991718 (21 April 2016); doi: 10.1117/12.2229469; https://doi.org/10.1117/12.2229469
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