16 September 2016 An optical tweezers, epi-fluorescence and microfluidic-setup for synchronization studies of glycolytic oscillations in living yeast cells
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Abstract
Yeast glycolysis is one of the best studied metabolic pathways and is a particularly good model system to study oscillatory behaviour, due to the tendency of yeast populations to synchronise their oscillations1. To resolve the question whether isolated yeast cells can oscillate, we studied yeast in micro-fluidic cells, under conditions that prevent cell-cell communication (low cell density, high flow rate). Thus, we could separate oscillations from synchronisation, which is not possible in typical population studies where a population average is monitored (i.e. where only synchronised cultures can be studied). After characterising the yeast oscillations in isolated cells, it is now important to allow cell-cell communication in the system to study the synchronisation characteristics.

A setup, consisting of an optical tweezers system and microfluidic devices coupled with fluorescence imaging was designed to perform a time dependent observation during artificially induced glycolytic oscillations. Multi-channel flow devices and diffusion chambers were fabricated using soft lithography. Automatized pumps controlled specific flow rates of infused glucose and cyanide solutions, used to induce the oscillations. Flow and diffusion in the microfluidic devices were simulated to assure experimentally the desired coverage of the solutions across the yeast cells, a requirement for time dependent measurements.

Using near infrared optical tweezers, yeast cells were trapped and positioned in array configurations, ranging from a single cell to clusters of various symmetries, in order to obtain information about cell-cell communications during the metabolic cycles.
Conference Presentation
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Martin Mojica-Benavides, Amin A. Banaeiyan, David D. van Niekerk, Jacky L. Snoep, Anna-Karin Gustavsson, Caroline B. Adiels, Mattias Goksör, "An optical tweezers, epi-fluorescence and microfluidic-setup for synchronization studies of glycolytic oscillations in living yeast cells", Proc. SPIE 9922, Optical Trapping and Optical Micromanipulation XIII, 992218 (16 September 2016); doi: 10.1117/12.2236208; https://doi.org/10.1117/12.2236208
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