Time-resolved and polarised microspectroscopy of thin films of bio- and nanomaterials

Hamid Soleimaninejad; Yuning Hong; Trevor A. Smith

doi:10.1117/12.2237084

12 October 2016 Time-resolved and polarised microspectroscopy of thin films of bio- and nanomaterials

Hamid Soleimaninejad, Yuning Hong, Trevor A. Smith

Proceedings Volume 9923, Physical Chemistry of Interfaces and Nanomaterials XV; 99230M (2016) https://doi.org/10.1117/12.2237084
Event: SPIE Nanoscience + Engineering, 2016, San Diego, California, United States

Abstract

We report on the use of time-resolved and polarised evanescent wave-induced fluorescence anisotropy measurements to probe molecular photophysics, motion and energy migration of fluorescent species in close proximity to a silica/film interface. In particular we show that the fluorescence decay and anisotropy of common fluorophores varies as a function of the plane of the fluorophore with respect to the interface, the distance from the interface, and as a function of position (using polarised EW imaging). We have applied time-resolved and polarised EW-induced fluorescence microspectroscopic measurements to dyes, thin polymer nanoparticle films and cells on silica surfaces, probing the variation in the photophysical dynamics within the films.

Citation Download Citation

Hamid Soleimaninejad, Yuning Hong, and Trevor A. Smith "Time-resolved and polarised microspectroscopy of thin films of bio- and nanomaterials", Proc. SPIE 9923, Physical Chemistry of Interfaces and Nanomaterials XV, 99230M (12 October 2016); https://doi.org/10.1117/12.2237084

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