12 October 2016 Time-resolved and polarised microspectroscopy of thin films of bio- and nanomaterials
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Abstract
We report on the use of time-resolved and polarised evanescent wave-induced fluorescence anisotropy measurements to probe molecular photophysics, motion and energy migration of fluorescent species in close proximity to a silica/film interface. In particular we show that the fluorescence decay and anisotropy of common fluorophores varies as a function of the plane of the fluorophore with respect to the interface, the distance from the interface, and as a function of position (using polarised EW imaging). We have applied time-resolved and polarised EW-induced fluorescence microspectroscopic measurements to dyes, thin polymer nanoparticle films and cells on silica surfaces, probing the variation in the photophysical dynamics within the films.
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Hamid Soleimaninejad, Hamid Soleimaninejad, Yuning Hong, Yuning Hong, Trevor A. Smith, Trevor A. Smith, } "Time-resolved and polarised microspectroscopy of thin films of bio- and nanomaterials", Proc. SPIE 9923, Physical Chemistry of Interfaces and Nanomaterials XV, 99230M (12 October 2016); doi: 10.1117/12.2237084; https://doi.org/10.1117/12.2237084
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