15 September 2016 Silver nanorod structures for metal enhanced fluorescence
Author Affiliations +
Fluorescence based detection is a commonly used methodology in biotechnology and medical diagnostics. Metalenhanced fluorescence (MEF) becomes a promising strategy to improve the sensitivity of fluorescence detection, where fluorophores coupling with surface plasmon on metallic structures results fluorescence enhancement. To apply the MEF methodology in real medical diagnostics, especially for protein or DNA microarray detection, a large area (e.g., slide glass, 75 × 25 mm2) with uniform metallic nanostructures is required. In this study, we fabricated a large area MEF substrates using oblique angle deposition (OAD), which is a single step, inexpensive large area fabrication method of nanostructures. To optimize the morphological effect, Ag-nanorods with various lengths were fabricated on the conventional slide glass substrates. Streptavidin-Cy5 dissolved in buffer solution with different concentration (100ng/ml ~ 100μg/ml) were applied to MEF substrates using a pipette, and the fluorescence signals were measured. The enhancement factor increased with the increase in length of Ag-nanorods and maximum enhancement factor ~ 91x was obtained from Ag-nanorods 750nm length compare to bare glass due to higher surface Plasmon effect.
Conference Presentation
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Mohsin Ali Badshah, Mohsin Ali Badshah, Xun Lu, Xun Lu, Jonghyun Ju, Jonghyun Ju, Seok-min Kim, Seok-min Kim, } "Silver nanorod structures for metal enhanced fluorescence", Proc. SPIE 9927, Nanoengineering: Fabrication, Properties, Optics, and Devices XIII, 992715 (15 September 2016); doi: 10.1117/12.2237464; https://doi.org/10.1117/12.2237464

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