3 October 2016 High-resolution synchrotron radiation-based phase tomography of the healthy and epileptic brain
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Abstract
Phase-contrast micro-tomography using synchrotron radiation has yielded superior soft tissue visualization down to the sub-cellular level. The isotropic spatial resolution down to about one micron is comparable to the one of histology. The methods, however, provide different physical quantities and are thus complementary, also allowing for the extension of histology into the third dimension. To prepare for cross-sectional animal studies on epilepsy, we have standardized the specimen’s preparation and scanning procedure for mouse brains, so that subsequent histology remains entirely unaffected and scanning of all samples (n = 28) is possible in a realistic time frame. For that, we have scanned five healthy and epileptic mouse brains at the ID19 beamline, ESRF, Grenoble, France, using grating- and propagation-based phase contrast micro-tomography. The resulting datasets clearly show the cortex, ventricular system, thalamus, hypothalamus, and hippocampus. Our focus is on the latter, having planned kainate-induced epilepsy experiments. The cell density and organization in the dentate gyrus and Ammon’s horn region were clearly visualized in control animals. This proof of principle was required to initiate experiment. The resulting three-dimensional data have been correlated to histology. The goal is a brain-wide quantification of cell death or structural reorganization associated with epilepsy as opposed to histology alone that represents small volumes of the total brain only. Thus, the proposed technique bears the potential to correlate the gold standard in analysis with independently obtained data sets. Such an achievement also fuels interest for other groups in neuroscience research to closely collaborate with experts in phase micro-tomography.
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Christos Bikis, Christos Bikis, Philipp Janz, Philipp Janz, Georg Schulz, Georg Schulz, Gabriel Schweighauser, Gabriel Schweighauser, Jürgen Hench, Jürgen Hench, Peter Thalmann, Peter Thalmann, Hans Deyhle, Hans Deyhle, Natalia Chicherova, Natalia Chicherova, Alexander Rack, Alexander Rack, Anna Khimchenko, Anna Khimchenko, Simone E. Hieber, Simone E. Hieber, Luigi Mariani, Luigi Mariani, Carola A. Haas, Carola A. Haas, Bert Müller, Bert Müller, } "High-resolution synchrotron radiation-based phase tomography of the healthy and epileptic brain", Proc. SPIE 9967, Developments in X-Ray Tomography X, 996706 (3 October 2016); doi: 10.1117/12.2237816; https://doi.org/10.1117/12.2237816
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