Automatic histology registration in application to x-ray modalities

Natalia Chicherova; Simone E. Hieber; Georg Schulz; Anna Khimchenko; Christos Bikis; Philippe C. Cattin; Bert Müller

doi:10.1117/12.2237322

21 October 2016 Automatic histology registration in application to x-ray modalities

Natalia Chicherova, Simone E. Hieber, Georg Schulz, Anna Khimchenko, Christos Bikis, Philippe C. Cattin, Bert Müller

Proceedings Volume 9967, Developments in X-Ray Tomography X; 996708 (2016) https://doi.org/10.1117/12.2237322
Event: SPIE Optical Engineering + Applications, 2016, San Diego, California, United States

Abstract

Registration of microscope images to Computed Tomography (CT) 3D volumes is a challenging task because it requires not only multi-modal similarity measure but also 2D-3D or slice-to-volume correspondence. This type of registration is usually done manually which is very time-consuming and prone to errors. Recently we have developed the first automatic approach to localize histological sections in μCT data of a jaw bone. The median distance between the automatically found slices and the ground truth was below 35 μm. Here we explore the limitations of the method by applying it to three tomography datasets acquired with grating interferometry, laboratory-based μCT and single-distance phase retrieval. Moreover, we compare the performance of three feature detectors in the proposed framework, i.e. Speeded Up Robust Features (SURF), Scale Invariant Feature Transform (SIFT) and Affine SIFT (ASIFT). Our results show that all the feature detectors performed significantly better on the grating interferometry dataset than on other modalities. The median accuracy for the vertical position was 0.06 mm. Across the feature detector types the smallest error was achieved by the SURF-based feature detector (0.29 mm). Furthermore, the SURF-based method was computationally the most efficient. Thus, we recommend to use the SURF feature detector for the proposed framework.

Conference Presentation

Citation Download Citation

Natalia Chicherova, Simone E. Hieber, Georg Schulz, Anna Khimchenko, Christos Bikis, Philippe C. Cattin, and Bert Müller "Automatic histology registration in application to x-ray modalities", Proc. SPIE 9967, Developments in X-Ray Tomography X, 996708 (21 October 2016); https://doi.org/10.1117/12.2237322

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