In Vitro Fluorescence Studies With Hematoporphyrin Derivative

Janine R. Shulok; Margaret H. Wade; Chi-Wei Lin

doi:10.1117/12.960180

17 March 1989 In Vitro Fluorescence Studies With Hematoporphyrin Derivative

Janine R. Shulok, Margaret H. Wade, Chi-Wei Lin

Author Affiliations +

Proceedings Volume 0997, Advances in Photochemotherapy; (1989) https://doi.org/10.1117/12.960180
Event: O-E/Fiber LASE '88, 1988, Boston, MA, United States

Abstract

The translocation and localization pattern of hematoporphyrin derivative (HpD) in human bladder tumor cells (MGH-U1) in culture was evaluated using fluorescence microscopy and interactive laser cytometry. The qualitative and quantitative fluorescence labelling patterns of HpD were compared with other fluorescent dyes specific for subcellular localization sites. Labelling of MGH-U1 cells with HpD initially showed fluorescence at the cell membrane. Later, diffuse perinuclear fluorescence was evident in these cells. This fluorescent staining pattern did not appear to be site-specific to either the mitochondria or endoplasmic reticulum but appeared to be generally distibuted to the cytomembranous structures of the cell. Lateral diffusion measurements indicated that the fluorescent components of HpD were bound to cellular lipids. These results suggest that HpD may localize in cells in vitro by a spontaneous diffusion process and be distributed to lipophilic sites within the cell.

Citation Download Citation

Janine R. Shulok, Margaret H. Wade, and Chi-Wei Lin "In Vitro Fluorescence Studies With Hematoporphyrin Derivative", Proc. SPIE 0997, Advances in Photochemotherapy, (17 March 1989); https://doi.org/10.1117/12.960180

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