28 September 2016 Polarization-correlation optical microscopy of anisotropic biological layers
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Abstract
The theoretical background of azimuthally stable method of Jones-matrix mapping of histological sections of biopsy of myocardium tissue on the basis of spatial frequency selection of the mechanisms of linear and circular birefringence is presented. The diagnostic application of a new correlation parameter – complex degree of mutual anisotropy – is analytically substantiated. The method of measuring coordinate distributions of complex degree of mutual anisotropy with further spatial filtration of their high- and low-frequency components is developed. The interconnections of such distributions with parameters of linear and circular birefringence of myocardium tissue histological sections are found. The comparative results of measuring the coordinate distributions of complex degree of mutual anisotropy formed by fibrillar networks of myosin fibrils of myocardium tissue of different necrotic states – dead due to coronary heart disease and acute coronary insufficiency are shown. The values and ranges of change of the statistical (moments of the 1st – 4th order) parameters of complex degree of mutual anisotropy coordinate distributions are studied. The objective criteria of differentiation of cause of death are determined.
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A. G. Ushenko, A. V. Dubolazov, V. A. Ushenko, Yu. A. Ushenko, M. Yu. Sakhnovskiy, V. N. Balazyuk, O. Khukhlina, K. Viligorska, A. Bykov, A. Doronin, I. Meglinski, "Polarization-correlation optical microscopy of anisotropic biological layers", Proc. SPIE 9971, Applications of Digital Image Processing XXXIX, 99712C (28 September 2016); doi: 10.1117/12.2237926; https://doi.org/10.1117/12.2237926
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