SpaceEye-1 earth observation satellite, developed by Satrec Initiative Co. Ltd., is a 300 kg scale spacecraft with high resolution electro-optical payload (EOS-D) which performs 1 m GSD, 12 km swath in low earth orbit. Metering structure of EOS-D is manufactured with Carbon Fiber Reinforced Plastic (CFRP). Due to the moisture emission from CFRP metering structure, this spaceborne electro-optical payload undergoes shrinkage after orbit insertion. The shrinkage of metering structure causes change of the distance between primary and secondary mirror. In order to compensate the moisture shrinkage effect, two types of thermal refocusing mechanism were developed, analyzed and applied to EOS-D. Thermal analysis simulating in-orbit thermal condition and thermo-elastic displacement analysis was conducted to calculate the performance of refocusing mechanism. For each EOS-D telescope, analytical refocusing range (displacement change between primary and secondary mirror) was 2.5 um and 3.6 um. Thus, the refocusing mechanism can compensate the dimensional instability of metering structure caused by moisture emission. Furthermore, modal, static and wavefront error analysis was conducted in order to evaluate natural frequency, structural stability and optical performance. As a result, it can be concluded that the refocusing system of EOS-D payload can perform its function in orbit.