Non-invasive detection and characterization of otitis media causing bacteria and bacterial biofilms through Raman spectroscopy and optical coherence tomography

Farzana Zaki; Andrea Locke; Sean Fitzgerald; Kavya Sudhir; Guillermo L. Monroy; Honggu Choi; Jungeun Won; Stephen A. Boppart; Anita Mahadevan-Jansen

doi:10.1117/12.2608410

7 March 2022 Non-invasive detection and characterization of otitis media causing bacteria and bacterial biofilms through Raman spectroscopy and optical coherence tomography

Farzana Zaki, Andrea Locke, Sean Fitzgerald, Kavya Sudhir, Guillermo L. Monroy, Honggu Choi, Jungeun Won, Stephen A. Boppart, Anita Mahadevan-Jansen

Author Affiliations +

Proceedings Volume PC11935, Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2022; PC1193502 (2022) https://doi.org/10.1117/12.2608410
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Otitis media or middle-ear infection is a widespread bacterial/viral disease. Antibiotic-resistant bacteria within biofilms emerge during chronic ear infections and are challenging to treat. We explored Raman spectroscopy (RS) and Optical Coherence Tomography (OCT) to identify and compare unique spectroscopic and microstructural features from primary otopathogenic bacteria in colony, planktonic, and biofilm forms, in vitro. RS was utilized to identify biochemical fingerprints and OCT was used to generate depth-resolved 2D and 3D images to compare refractive indices and optical attenuation coefficients. A combined RS-OCT system will enable real-time visualization and diagnosis of bacterial OM at the point-of-care.

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Farzana Zaki, Andrea Locke, Sean Fitzgerald, Kavya Sudhir, Guillermo L. Monroy, Honggu Choi, Jungeun Won, Stephen A. Boppart, and Anita Mahadevan-Jansen "Non-invasive detection and characterization of otitis media causing bacteria and bacterial biofilms through Raman spectroscopy and optical coherence tomography", Proc. SPIE PC11935, Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2022, PC1193502 (7 March 2022); https://doi.org/10.1117/12.2608410

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KEYWORDS

Optical coherence tomography

Bacteria

Raman spectroscopy

Ear

Diagnostics

Remote sensing

Real time imaging

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