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7 March 2022 Laser speckle contrast imaging of cerebral blood flow using picosecond pulsed laser illumination
Faraneh Fathi, Siavash Mazdeyasna, Mehrana Mohtasebi, Dara Singh, Xuhui Liu, Chong Huang, Samaneh Rabienia Haratbar, Lei Chen, Guoqiang Yu
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Proceedings Volume PC11944, Multiscale Imaging and Spectroscopy III; PC119440G (2022) https://doi.org/10.1117/12.2610178
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Laser speckle contrast imaging (LSCI) illuminates continuous-wave (CW) laser light on tissue surface. We assembled an integrated LSCI system combining a CW laser at 785 nm and a picosecond pulsed laser at 775 nm. A CMOS camera collected images from mouse head with intact skull. The pulsed laser with engineered diffuser captured more details of brain vessels compared to the CW laser with glass diffusers. The consecutive ligations of left and right common carotid arteries resulted in significant CBF reductions. This research lays the ground to develop multimodal imaging systems integrating LSCI and other imaging techniques with shared pulse illuminations.
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Faraneh Fathi, Siavash Mazdeyasna, Mehrana Mohtasebi, Dara Singh, Xuhui Liu, Chong Huang, Samaneh Rabienia Haratbar, Lei Chen, and Guoqiang Yu "Laser speckle contrast imaging of cerebral blood flow using picosecond pulsed laser illumination", Proc. SPIE PC11944, Multiscale Imaging and Spectroscopy III, PC119440G (7 March 2022); https://doi.org/10.1117/12.2610178
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KEYWORDS
Laser speckle contrast imaging
Pulsed laser operation
Picosecond phenomena
Cerebral blood flow
Imaging systems
Continuous wave operation
Diffusers
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