Label-free measurement of human cells biophysical properties with a methodology combining microfluidic devices and digital holographic microscopy

Erik Bélanger; Gabrielle Jess; Sara Mattar; Mohamed Haouat; Émile Rioux-Péllerin; Marie-Ève Crochetière; Jean-Xavier Giroux; Pierre Marquet

doi:10.1117/12.2610473

7 March 2022 Label-free measurement of human cells biophysical properties with a methodology combining microfluidic devices and digital holographic microscopy

Erik Bélanger, Gabrielle Jess, Sara Mattar, Mohamed Haouat, Émile Rioux-Péllerin, Marie-Ève Crochetière, Jean-Xavier Giroux, Pierre Marquet

Author Affiliations +

Proceedings Volume PC11955, Microfluidics, BioMEMS, and Medical Microsystems XX; PC1195505 (2022) https://doi.org/10.1117/12.2610473
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Biophysical properties (BPs) of a cell depend drastically on its physiological or pathological state. Thus, being able to accurately and non-invasively measure a set of cell BPs, that reflect these cellular states, is of major importance. To this end, we propose an approach that combines customized fluidic devices with digital holographic microscopy (DHM). Specifically, we have developed several low-cost 3D-printed millifluidic devices which when combined with DHM allow to measure in a controlled physiological environment specific cell BPs including intracellular refractive index, absolute cell volume, membrane flickering as well as cell elasticity and viscosity moduli.

Conference Presentation

Citation Download Citation

Erik Bélanger, Gabrielle Jess, Sara Mattar, Mohamed Haouat, Émile Rioux-Péllerin, Marie-Ève Crochetière, Jean-Xavier Giroux, and Pierre Marquet "Label-free measurement of human cells biophysical properties with a methodology combining microfluidic devices and digital holographic microscopy", Proc. SPIE PC11955, Microfluidics, BioMEMS, and Medical Microsystems XX, PC1195505 (7 March 2022); https://doi.org/10.1117/12.2610473

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available